Year: 2015 | Month: September | Volume 5 | Issue 3
Recovery of Different Cumulus Oocyte Complex (COC) Grades from Bovine Ovaries by Aspiration Method
Abstract:
The present study was undertaken to evaluate the aspiration method for recovery of cumulus oocyte complexes (COCs) from
bovine ovaries at slaughter. A total of 829 cattle ovaries were collected from slaughter house, out of which 2,935 COCs were
aspirated out. The mean number of COCs recovered was 3.54 per ovary. The COCs recovered were graded variously as A, B,
C and D grades. Grade ‘A’ and ‘B’ COC’s were 38.77% and 27.02% respectively, and the average culture grade (Grade A and
B COC’s together) recovery was 65.79%, representing 2.33 COCs per ovary. The number and percentage recovery of A and B grade COCs were higher than that of C (18.13%; 0.64/ovary) and D (16.08%; 0.57/ovary) grades. The aspiration method adopted in the present study was found to be effi cient for recovery of ample amount of COCs and yield good quality culturable oocytes for subsequent in vitro studies. The aspiration method also allows greater visual assessment of follicles, their selection and assortment for quality COC yield.
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